What is the meaning of biuret?

: a chemical reaction that produces a violet or purple color when biuret or most proteins are exposed to a reagent of copper sulfate in alkaline solution.

Is biuret an acid or base?

Biuret is a chemical compound with the chemical formula [H2NC(O)]2NH. It is a white solid that is soluble in hot water. The term “biuret” also describes a family of organic compounds with the functional group -(HN-CO-)2N-. Biuret.

Related compounds urea, triuret, cyanuric acid

What is biuret test for urea?

A biochemical test to detect proteins in solution, named after the substance biuret (H2NCONHCONH2), which is formed when urea is heated. Sodium hydroxide is mixed with the test solution and drops of 1% copper(II) sulphate solution are then added slowly.

Is called biuret reagent?

The Biuret reagent is made of sodium hydroxide (NaOH) and hydrated copper(II) sulfate, together with potassium sodium tartrate, the latter of which is added to chelate and thus stabilize the cupric ions. A tri- or tetra-dentate chelation with the peptide nitrogen produces the characteristic color.

How is biuret pronounce?

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What color is biuret solution?

pale blue We used Biuret’s reagent to detect the presence of proteins in solution. The reagent is pale blue when pure, but when mixed with proteins, the resulting reaction produces a pale purple color.

Why is biuret purple?

The normal color of biuret reagent is blue. The reagent turns violet in the presence of peptide bonds — the chemical bonds that hold amino acids together. The reagent’s copper ions, with a charge of +2, are reduced to a charge of +1 in the presence of peptide bonds, causing the color change.

How do you stop biuret?

CONTROLLING BIURET IN UREA PRODUCTION (English) Disclosed is a novel method of controlling the formation of biuret in urea production, and particularly reducing, preventing or reversing such formation. This is accomplished by adding liquid ammonia to a urea aqueous stream.

What are the advantages of biuret method?


Method Advantages Example assay reagents
Biuret methods: Protein-copper chelation and secondary detection of reduced copper Compatibility with most surfactants (detergents) Linear response curve (R2 > 0.95) Less protein–protein variation than the Coomassie dye–based assays BCA Assays Lowry Assays

Does urea give Biuret test?

An Overview of Biuret Test. Biuret is a compound produced by heating urea at 180 ℃. Biuret test is the name of a chemical test which utilises the Biuret reagents which contains a 1% solution of Copper II sulphate (CuSO₄).

Why is BSA used in Biuret test?

An aqueous solution of bovine serum albumin (BSA) is commonly used as a standard in testing because of its stability in testing, low cost, and is readily available as a byproduct from bovine blood.

How does Biuret test detect urea?

Hint: Biuret test is a test performed to detect the presence of peptide bonds in the compounds. In the case of urea, the amino acids are being analyzed and thus, the protein structure and the peptide bonds associated with it are determined.

What color is a negative biuret test?

RESULTS: Denim-blue = negative. Lavender = positive. Water plus Biuret’s reagent is a negative control for the protein test. It demonstrates a negative test result (no protein present).

What is the principle behind biuret test?

The biuret method is based on the fact that proteins (and, as a rule, all substances containing two or more peptidic bonds) react with copper to form a colored complex whose absorption (λmax= 454 nm), in the presence of excess copper, is proportional to the amount of protein present.

Is biuret solution harmful?

Not a dangerous substance or mixture according to the Globally Harmonised System (GHS). Inhalation May be harmful if inhaled. Causes respiratory tract irritation. Skin May be harmful if absorbed through skin.

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